Blar i forfatter "Priyadarshi, Anish"

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    • Chip-based multimodal super-resolution microscopy for histological investigations of cryopreserved tissue sections 

      Villegas, Luis; Dubey, Vishesh Kumar; Nystad, Mona; Tinguely, Jean-Claude; Coucheron, David Andre; Dullo, Firehun Tsige; Priyadarshi, Anish; Acuna Maldonado, Sebastian Andres; Ahmad, Azeem; Mateos, Jose M.; Barmettler, Gery; Ziegler, Urs; Birgisdottir, Åsa Birna; Hovd, Aud-Malin Karlsson; Fenton, Kristin Andreassen; Acharya, Ganesh; Agarwal, Krishna; Ahluwalia, Balpreet Singh (Journal article; Tidsskriftartikkel; Peer reviewed, 2022-02-24)
      Histology involves the observation of structural features in tissues using a microscope. While diffraction-limited optical microscopes are commonly used in histological investigations, their resolving capabilities are insufficient to visualize details at subcellular level. Although a novel set of super-resolution optical microscopy techniques can fulfill the resolution demands in such cases, the ...

    • Fluorescence fluctuation-based super-resolution microscopy using multimodal waveguided illumination 

      Opstad, Ida Sundvor; Hansen, Daniel Henry; Acuña Maldonado, Sebastian Andres; Ströhl, Florian; Priyadarshi, Anish; Tinguely, Jean-Claude; Dullo, Firehun Tsige; Dalmo, Roy Ambli; Seternes, Tore; Ahluwalia, Balpreet Singh; Agarwal, Krishna (Journal article; Tidsskriftartikkel; Peer reviewed, 2021-07-19)
      Photonic chip-based total internal reflection fluorescence microscopy (c-TIRFM) is an emerging technology enabling a large TIRF excitation area decoupled from the detection objective. Additionally, due to the inherent multimodal nature of wide waveguides, it is a convenient platform for introducing temporal fluctuations in the illumination pattern. The fluorescence fluctuation-based nanoscopy technique ...

    • Photonic-chip: a multimodal imaging tool for histopathology 

      Villegas, Luis; Dubey, Vishesh Kumar; Tinguely, Jean-Claude; Coucheron, David Andre; Priyadarshi, Anish; Acuña Maldonado, Sebastian Andres; Agarwal, Krishna; Mateos, Jose M; Nystad, Mona; Hovd, Aud-Malin Karlsson; Fenton, Kristin Andreassen; Ahluwalia, Balpreet Singh (Conference object; Konferansebidrag, 2021-04)
      We propose the photonic-chip as a multimodal imaging platform for histopathological assessment, allowing large fields-of-view across diverse microscopy methods including total internal reflection fluorescence and single-molecule localization.

    • A transparent waveguide chip for versatile total internal reflection fluorescence-based microscopy and nanoscopy 

      Priyadarshi, Anish; Wolfson, Deanna; Ahmad, Azeem; Jayakumar, Nikhil; Dubey, Vishesh Kumar; Tinguely, Jean-Claude; Ahluwalia, Balpreet Singh; Murugan, Ganapathy Senthil (Journal article; Tidsskriftartikkel; Peer reviewed, 2021-08-20)
      Total internal reflection fluorescence (TIRF) microscopy is an imaging technique that, in comparison to confocal microscopy, does not require a trade-off between resolution, speed, and photodamage. Here, we introduce a waveguide platform for chip-based TIRF imaging based on a transparent substrate, which is fully compatible with sample handling and imaging procedures commonly used with a standard ...